Mouse intragastric infusion (iG) model

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Intragastric infusion of nutrients in cattle.

1. A method of continuous alimentation of cattle by total infusion of nutrients has been developed. Friesian steers within the weight range 100-400 kg live weight and dairy cows were used. 2. A multi-channel peristaltic pump was used to infuse solutions of volatile fatty acids (VFA), minerals, and buffer through a cannula in the rumen and a casein-vitamin solution into the abomasum. 3. The meth...

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Nitrogen and purine metabolism at varying energy and protein supplies in sheep sustained on intragastric infusion.

Wether sheep were fitted with rumen fistulas and polyethylene tubes to the abomasum and were given all nutrients by intragastric infusion. In Expt 1 volatile fatty acids (VFA) were given at 340, 450 and 630 kJ gross energy (GE)/kg metabolic weight (W0-75) and protein at 0, 150, 300, 600, 900 and 1500 mg nitrogen/kg W0-75. In Expt 2 VFA were infused at 450 kJ GE/kg W0-75 and protein at 0 and 300...

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Gastric branch vagotomy and gastric emptying during and after intragastric infusion of glucose.

The effect of gastric branch vagotomy (GVX) on the gastric emptying of glucose was evaluated during two phases of emptying control: as the stomach fills and in the postload period. GVX and control rats received a series of intragastric glucose infusions (1.0 ml/min) through indwelling gastric fistulas. In experiment 1, gastric samples were withdrawn either immediately after the offset of 9- or ...

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ژورنال

عنوان ژورنال: Nature Protocols

سال: 2012

ISSN: 1754-2189,1750-2799

DOI: 10.1038/nprot.2012.014